1.1孟德尔豌豆杂交实验（一）课件 2025—2026学年高一下学期生物人教版必修2

第1节 孟德尔的豌豆杂交实验（一） 第 1 章  遗传因子的发现 现代遗传学之父---孟德尔 知识梳理 遗传术语 性状：生物体的形态特征和生理特性。 相对性状：一种生物的同一种性状的不同表现类型 显性性状：F1中表现出来的性状，如高茎。 隐性性状：F1中未表现出来的性状，如矮茎。 性状分离：杂种后代中，同时出现显性性状和隐性性状的现象。 显性遗传因子：大写，如D。 隐性遗传因子：小写，如d。 纯合子：遗传因子组成相同的个体，高茎DD，矮茎dd。 杂合子：遗传因子（基因）组成不同的个体，高茎Dd。 知识梳理 性状 相对性状 显性性状 隐性性状 显性遗传因子（D） 隐性遗传因子（d） 性状分离 遗传因子 纯合子（DD、dd） 杂合子（Dd） 自交后代不出现 自交后代出现 决定 决定 决定 同时出现显、隐性性状 知识梳理 概 念 应 用 杂交 基因型 的 雌雄个体相交。 ①通过杂交将不同的优良性状集中到一起，得到新品种（ 育种） ②通过后代性状分离比判断个体显隐性。 （如：分离比为3﹕1 ，则3为 性，1为 性） 自交 基因型 的 雌雄个体相交。 ①连续自交可不断提高种群中 的比例 ②根据后代是否发生性状分离，可用于植物 的鉴定 测交 F1与 。相交。 ①根据后代是否发生性状分离，测定F1的基因组成， 或者用于动、植物 的鉴定 ②验证 定律解释的正确性 不同 相同 杂交 显 隐 纯合子 纯合子、杂合子 隐性纯合子 纯合子、杂合子 基因分离 符号 P F1 F2 × ⊗ ♀ 含义 亲本 ________ ________ 杂交 _____ 母本 父本 子一代　 子二代　 自交　 概念辨析 ♂ 遗传实验中常用符号及含义 正交： 在具有相对性状的遗传实验中，若将显性个体作母本，隐性个体作父本的杂交方式称为正交； 反交： 将显性个体作父本，隐性个体作母本的杂交方式就为反交。 正交与反交是相对的，若我们假设其中一种为正交，那么另一种就是反交。 知识梳理 1 2 3 豌豆用作遗传实验材料的优点 一对相对性状的杂交实验 对分离现象的解释 4 5 6 性状分离比的模拟实验 对分离现象解释的验证 分离定律 1 豌豆用作遗传实验材料的优点 优点①自花传粉、闭花授粉 豌豆花是两性花，在未开放时，它的花粉会落到同一朵花的雌蕊的柱头上，从而完成受粉，这种传粉方式叫作自花传粉，也叫自交。 避免了外来花粉的干扰，所以豌豆在自然状态下一般都是纯种，用豌豆做人工杂交实验（图1-1），结果既可靠，又容易分析。 1 豌豆用作遗传实验材料的优点 优点②具有易于区分的性状（trait） 性状 相对性状 图1-2 豌豆的7对相对性状示意图 1 豌豆用作遗传实验材料的优点 优点③豌豆花大，便于人工进行异花授粉（杂交） 图1-1 人工异花传粉示意图 思考： 1.什么是父本和母本？ 2.对母本去雄在什么时候？为什么？ 3.为何母本去雄后和传粉后都要套上纸袋？ 父本：提供花粉 母本：接受花粉 雄蕊未成熟时；避免自花传粉。 避免外来花粉的干扰。 ♀ ♂ 1 豌豆用作遗传实验材料的优点 去雄 套袋 传粉 套袋 先除去未成熟花的全部雄蕊。 套上纸袋，防止外来花粉干扰。 人工异花传粉流程 雌蕊成熟时将另一植株的花粉（撒）在去雄的雌蕊的柱头上。 保证杂交得到的种子是人工授粉后所结。 图1-1 人工异花传粉示意图 假说演绎法 研究方法： 观察现象，提出问题： 分析问题，提出假说： 根据假说，演绎推理： 设计实验，验证假说： 归纳综合，总结规律： 2 一对相对性状的杂交实验 高茎 高茎 高茎 高茎 高茎 矮茎 矮茎 1.为什么子一代都是高茎而没有矮茎的呢？ 2.为什么子一代没有矮茎的，而子二代又出现了矮茎的呢？ 观察现象，提出问题 显性性状： 两个具有相对性状的纯合亲本杂交，F1中表现出来的性状， 隐性性状： F1中未表现出来的性状， 性状分离： 杂种后代中，同时出现显性性状和隐性性状的现象。 如高茎。 如矮茎。 图1-3 高茎豌豆和矮茎豌豆的杂交实验示意图 高茎 高茎 高茎 矮茎 矮茎 2 一对相对性状的杂交实验 子二代中出现3∶1的性状分离比是偶然的吗？ 3 ∶ 1 观察现象，提出问题 表1-1孟德尔还对豌豆的其他6对相对性状进行了杂交实验 性状 F2的表现 显性 隐性 显性∶隐性 茎的高度 高茎 787 矮茎 277 2.84 ∶ 1 种子形状 圆粒 5 474 皱粒 1850 2.96 ∶ 1 子叶颜色 黄色 6 022 绿色 2001 3.01 ∶ 1 花的颜色 红色 705 白色 224 3.15 ∶ 1 豆荚形状 饱满 882 不饱满 299 2.95 ∶ 1 豆荚颜色（未成熟） 绿色 428 黄色 152 2.82 ∶ 1 花的位置 腋生 651 顶生 207 3.14 ∶ 1 对分离现象的解释 3 分析问题，提出假说 （1）生物的性状是由遗传因子（hereditary factor）决定的。 显性遗传因子（大写，如D） 隐性遗传因子（小写，如d） 显性性状 隐性性状 注意： 控制一对相对性状的遗传因子一定要用同一字母 决定 决定 对分离现象的解释 3 分析问题，提出假说 （2）在体细胞中，遗传因子是成对存在的。 如：纯种高茎豌豆的遗传因子DD， 纯种矮茎豌豆的遗传因子dd。 纯合子： 遗传因子组成相同的个体。纯合子能稳定遗传，自交后代不会发生性状分离。 杂合子： 遗传因子（基因）组成不同的个体。杂合子不能稳定遗传，自交后代会发生性状分离。 如：F1中的高茎豌豆的遗传因子Dd。 对分离现象的解释 3 分析问题，提出假说 （3）生物体在形成生殖细胞——配子时，成对的遗传因子彼此分离，分别进入不同的配子中。配子中只含有每对遗传因子中的一个。 纯合子只产生一种配子 杂合子产生两种配子 DD D Dd dd D : d = 1 : 1 d （4）受精时，雌雄配子的结合是随机的。 对分离现象的解释 3 分析问题，提出假说 图1-4 高茎豌豆与矮茎豌豆杂交实验的分析图解 P 配子 F1 F1 配子 F2 高茎 高茎 高茎 高茎 高茎 高茎 高茎 矮茎 DD DD dd dd 矮茎 Dd Dd Dd D d Dd D d 1 : 2 : 1 × × Dd D d 4 对分离现象解释的验证 测交法： 让F1和隐性纯合子杂交，用来测定F1的遗传因子组成。 问题1： 你们认为测交实验关键要验证什么？ 问题2： 测交实验的设计思路是什么？ 验证：F1（Dd）是否产生了两种配子（D和d），且比例是1：1。 隐性纯合子（dd）只产生一种含有隐性遗传因子（d）的配子，测交后代的性状表现应该由F1产生的配子种类及比例讹赖决定，因此，可以通过观察测交后代的性状表现及比列来推测F1产生的配子种类和比例额，从而推出的F1遗传因子组成。 4 对分离现象解释的验证 P 配子 F1 高茎 高茎 矮茎 Dd dd d × 测交 杂种子一代 隐性纯合子 D d Dd dd 矮茎 1 : 1 演绎推理，验证假说 测交实验的结果： 在得到的166株后代中，87株是高茎的，79株是矮茎的，高茎与矮茎植株的数量比接近1∶1。 得出结论： 测交实验结果与预测相符，表明F1产生了两种配子（D和d），且比例是1：1。 假说正确，即F1（Dd）在形成配子时，成对的遗传因子彼此分离，分别进入不同的配子。 分离定律 5 假说演绎法 研究方法： 观察现象，提出问题： 分析问题，提出假说： 根据假说，演绎推理： 设计实验，验证假说： 归纳综合，总结规律： 为什么F2中出现3∶1的性状分离比？ F1在产生配子时，成对的遗传因子彼此分离，分别进入不同的配子中。 如果F1在形成配子时，成对的遗传因子彼此分离，分别进入不同的配子中，那么F1与隐性纯合子杂交产生的后代应该是高茎与矮茎之比是1∶1。 测交实验结果与演绎推理预测相符。 孟德尔分离定律 分离定律 5 孟 德 尔 第 一 定 律， 又 称 分离定律（law of segregation） 在生物的体细胞中，控制同一性状的遗传因子成对存在，不相融合；在形成配子时，成对的遗传因子发生分离，分离后的遗传因子分别进入不同的配子中，随配子遗传给后代。 适用范围 一对相对性状的遗传 进行有性生殖的真核生物 6 性状分离比的模拟实验 本实验用甲、乙两个小桶分别代表雌、雄生殖器官，甲、乙小桶内的彩球分别代表雌、雄配子，用不同彩球的随机组合，模拟生物在生殖过程中，雌、雄配子的随机结合。 【实验原理】 D d 显性遗传因子 隐性遗传因子 6 性状分离比的模拟实验 【目的要求】 通过模拟实验，理解遗传因子的分离、配子的随机结合与性状之间的数量关系，体验孟德尔的假说。 【材料用具】 两个小桶，分别标记甲、乙；两种大小相同、颜色不同的彩球各20个，一种彩球标记D，另一种彩球标记d ；记录用的纸和笔。 （3）分别从两个桶内随机抓取一个彩球，组合在一起，记下两个彩球的字母组合。 6 性状分离比的模拟实验 【方法步骤】 （1）在甲、乙两个小桶中放入两种彩球各10个。 （2）摇动两个小桶，使小桶内的彩球充分混合。 （4）将抓取的彩球放回原来的小桶内，摇匀。 （5）按步骤（3）和（4）重复做30次以上。 确保两种类型的配子数目一样 模拟雌、雄配子的随机结合 确保样本数目足够多。雌、雄配子结合的机会相等。 6 性状分离比的模拟实验 次数 甲 乙 组合 1 2 3 4 5 ...... 29 30 合计次数 D 次 d 次 D 次 d 次 DD 次 Dd 次 dd 次 统计数量比 D： d = D： d = DD：Dd：dd = 性状分离比的模拟实验记录表 6 性状分离比的模拟实验 【结果和结论】 1．统计实验结果：彩球组合有几种？每种组合的数量是多少？计算彩球组合类型之间的数量比。设计表格，记录实验结果。 2．统计全班的实验结果，求平均值。计算彩球组合类型之间的数量比。 3．两个彩球的组合代表什么？ 4．结论：__________________________________________________ _________________________________________________。 模拟雌、雄配子的随机结合 6 性状分离比的模拟实验 讨论: 1．将每个小组的实验结果与全班总的实验结果作比较，你有什么发现？如果孟德尔当时只对F2中10株豌豆的性状进行统计，他还能正确地解释性状分离现象吗？ 与每个小组的实验结果相比，全班总的实验结果更接近预测的结果，即彩球组合类型比DD:Dd;dd=1：2：1；彩球代表的显性与隐性类型的数量比为3：1。因为实验统计的样本数量越大，越接近统计规律。 如果孟德尔当时只对F2中10株豌豆的性状进行统计，那么他很难正确地解释性状分离现象。因为实验统计的样本数量足够大，是孟德尔能够正确分析实验结果的前提条件之一。只对10株豌豆的性状进行统计，会出现较大的误差。 讨论: 2．将模拟实验的结果与孟德尔的杂交实验结果相比较，你认为孟德尔的假说是否合理？ 合理。因为甲乙两个小桶内的彩球分别代表孟德尔杂交实验中的雌雄配子，分别从两个桶内随机抓取一个彩球进行组合，实际上是模拟雌雄配子的随机结合，统计的样本数量也足够大，出现了3：1的结果。但孟德尔提出的假说是否正确还需要实验来验证。 6 性状分离比的模拟实验 懂了 0310601010227/coverImage.png 0310601010227/favicon.ico 0310601010227/index.html 0310601010227/meta.json { "title":"豌豆果实的结构", "bizId": "0310601010227", "subject": "science", "knowledge": "life_science", "mediaSubType": "3d", "dependencies":["babylonjs"], 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